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10.1016/j.ccell.2018.10.005 119 JungC
Steady-state kinetics were measured using a pyrophosphate assay for the apo and AdoCbl-bound dark/light states of the Ab DPcob protein, as well as the truncated DGC domain only (Fig

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RNA isolation and quantitative real-time RT-PCR Total RNA was isolated from Jurkat T cell using Trizol (Invitrogen, Carlsbad, CA, USA)
