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Glutathione is a naturally occurring antioxidant found in every cell of the body, primarily produced in the liver

Three pairs of monopolar needles were stabbed 3 mm deep into the tail 10, 60, and 100 mm caudal to the tail base

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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch