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The reaction mixture, which had a total volume of 25 l, contained 3 l of total RNA, 4 l of 5x Trans Amp buffer, 0.25 l of reverse transcriptase, 0.5 l of each primer, 12.5 l of 2x Quantitect SYBR green PCR master mix, and 8.25 l of RNase-free water

Le DT, Durham JN, Smith KN et al (2017) Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade

doi:10.1016/j.biomaterials.2015.11.061 67

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