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Cycling Strategy: 6 weeks on, 2 weeks off maintains receptor sensitivity
doi: 10.1111/ele.12482
this is critical since these organelles are major contributors to free radical generation
Cells were lysed with DPBS solution containing 1% Triton X-100 (FUJIFILM Wako pure chemical, Osaka, Japan), and then fluorescence intensity was measured at 515 nm (excitation: 490 nm) using a SpectraMax M2e microplate reader (Molecular Devices, San Jose, CA)
