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5 L of each sample was analysed on a Acquity UPLC HSS-T3 column (50 2.1 mm, 1.8 m, Waters, MA, USA) by using gradient elution of 0.1% formic acid ( v / v ) in water as mobile phase A and 0.1% formic acid ( v / v ) in acetonitrile as mobile phase B, with a flow rate of 0.4 mL/min with the following gradient: 00.5 min (0% B), 0.8 min (15% B), 1.2 min (80% B), 1.4 min (80% B), 1.5 min (0% B), 2 min (0% B)

Furthermore, chitosan has demonstrated intrinsic anti-inflammatory properties by inhibiting tumor necrosis factor alfa (TNF-) secretion [34]

High levels of vesicles are found in the NMJ of patients with ALS, suggesting dysfunctional axonal transport [286]

Reactive oxygen (ROS) and nitrogen species arising from this stress damage nucleic acids, lipids, and proteins, leading to the cell and tissue dysfunction