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Serum iodide (I-) enters the follicular cell via a Na/I symporter against a concentration gradient, using the Na/K ATPase to generate the sodium gradient to drive iodide trapping. Thyroglobulin (TG) is exocytosed, and iodide is transported via the apical membrane anion transporter Pendrin , to the amorphous acellular colloid portion of the thyroid

After 18 hours, luminol and HPI were added to the wells, both to 250 M final concentration
