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Description
Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken
doi: 10.1186/s12986-022-00696-y 96
WQC, SSL, CC, and JC performed the experiments
doi:10.1023/B:BEBM.0000038164.49947.8c PMID: 15455129 Anisimov VN, Khavinson VKh, Provinciali M, et al
