glutathione sepharose wiki File:Glutathione structure.png Pull-down assays
Description
[DOI] [PubMed] [Google Scholar] 80.Shimidzu N., Goto M., Miki W
Exp Neurol 311:88105
22 | Add 0.5 ml of Pop-Culture (final 10 % vol/vol) and 100 l of 50 mM 2-mercaptoethanol (final 1 mM) to the resuspended pellet (from Step 21)
Notably, both DFO and ammonium ferric citrate enhance erastin-induced cell death, implying that although systemic iron overload exists in FRDA, cytosolic iron may still be limiting, and iron metabolism is dysregulated, contributing to cellular vulnerability
