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The pH-dependent aquatic toxicities of ionizable compounds have been of concern 3,4,5,6,10 , because these values affect risk assessment

Interestingly, the mesenchymal shift by Day 45 suggests greater cellular complexity at later stages, potentially facilitating iHLO use in modeling liver pathologies such as fibrosis, cirrhosis, and liver remodeling, as indicated by increased expression of hepatic stellate cell markers such as DES and VIM 33,34

Segmental defect healing was evaluated with bi-weekly radiographs and histological examinations

Furthermore, our results were also consistent with observations reported by Raymond et al