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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)
It is critical that patients report any unusual symptoms promptly to their healthcare provider
SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism
As shown in Supplementary Figure 1, we did not observe a significant difference in the cell number between the control and ABT263 treatment group
