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Pan, M., Wang, X., Zhao, Y., Liu, W
Alexa Fluor 594 nm donkey anti-rabbit IgG secondary antibody (1:800) (Jackson ImmunoResearch Laboratory Inc.), 1.5 g/mL Dapi and 3 M BODIPY 3922 diluted in blocking buffer was applied to samples for 2 h at room temperature

You start at the full therapeutic dose from the first injection and maintain it for all 4

Circulation 113 , 11141122 (2006)
