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ALA can also enhance the developmental potential of oocytes in the mouse by reducing the level of ROS [96]

Following 3 10min washes with 1ml 0,2% TBS-Gelatin and 1 wash with regular TBS the slides were mounted using soft set mounting medium with DAPI (Vectashield) and sealed with nail polish

Treatment of the medium with extremes of pH (1.5 or 12) resulted in significant activation of TGF- as shown by radio-receptor assays, while mild acid treatment (pH 4.5) yielded only 20-30% of the activation achieved by pH 1.5