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24 h after the treatment, cells were fixed with 10% trichloroacetic acid, extensively washed, then incubated with the dye, washed and finally resolubilized in 10 mM Tris base, prior to scanning the plates at 565 and 690 nm (main signal and background, respectively)
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Blockade of MCT1 dramatically reduced lactate levels with modest effects on glucose consumption by DC
Murray J
