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The pMD-19T (Simple) vector, DNA ligation kit, restriction enzymes Eco R I and Not I were purchased from TaKaRa Biomedical Technology (Japan) Co., Ltd

doi: 10.1016/j.cca.2020.05.041

*Correspondence: Xi Cheng, [email protected] Niu, [email protected] These authors have contributed equally to this work and share first authorship This article was submitted to Dementia and Neurodegenerative Diseases, a section of the journal Frontiers in Neurology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The extract of pruriens free of LDs contained catechols and polyphenols, which upregulated the expression of NO-producing genes