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The experiments were carried out according to ARRIVE guidelines and the US National Institutes of Health, by the approval of the Research and Ethics Committee of Riphah Institute of Pharmaceutical Sciences, Riphah International University (Reference no: REC/RIPS/2022/06)
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Animals mounted on 2% agarose pads and 20 mM sodium azide were imaged under the same parameters (63x objective, Z-stack and exposure time) on the Zeiss Axioimager Z1
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