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293A cells were then transfected with the adenoviral expression clones using Lipofectamine 2000 (Thermo Fisher Scientific, #11668019), and crude adenoviral lysates were collected 710 days post transfection

ATPase activity assays confirmed that coumarin stimulates ATP hydrolysis by P-glycoprotein (P-gp), indicating that coumarin acts as a substrate for it, and the nanoparticles were shown to inhibit P-gp-mediated efflux, enhancing drug retention in cells

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