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Chromatographic analysis of the GSH-OPA abduct was performed using reverse-phase chromatography on Agilent 1200 HPLC (Agilent Technologies, Stockport, UK) with G1311a Quaternary pump, G1367a Autosampler (5 L injection), G1316a Column Compartment (37 C) G1321B Fluorescent light detector, fitted with Phenomenex Gemini C18 100X2 mm and an additional guard column (Phenomenex, Macclesfield, UK) and a stepwise solvent gradient of 25 mM NaH 2 PO 4 (pH 6.0) and acetonitrile as the mobile phase

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The peptide reconstitution calculator at SeekPeptides handles this math instantly, but understanding the underlying formula means you can verify any calculation independently

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