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Partial oxygenation results in a Hb conformational change that produces a dramatic increase in the rate of autoxidation and the affinity of Hb for the RBC membrane (Cao et al., Extracellular hemoglobin as a source for oxidative stress Extracellular Hb, whether originating from hemolysis of RBCs or the infusion of cell-free Hb-based blood substitutes, can be a major source for oxidative stress

(PubMed) Whelan AM, Jurgens TM, Naylor H

Of these, apolipoprotein L1 and glutathione peroxidase 3 (GPX3) displayed the strongest positive and inverse associations, respectively