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10.1016/j.pbb.2016.01.010 29 GraeberM

The tight junction is composed of several transmembrane and cytosolic proteins, including occludin, claudins, zonula occludens (ZOs), tricellulin, cingulin, and junctional adhesion molecules (JAM), which interact with each other, as well as with the cytoskeleton, and form a complex architecture 27

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The primary antibody was removed, and the sections were washed with PBS three times, each wash lasting 5 min