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After centrifugation, the supernatant was used to determine the concentrations of GSH and GSSG using the method described by Julicher, et al.29 Frozen biopsies for myeloperoxidase (MPO) analyses were ground with a mortar and pastel cooled in liquid nitrogen, and resuspended in 100 L ice-cold PBS containing 10 L/mL Protease Inhibitor Cocktail (Sigma-Aldrich, Zwijndrecht, the Netherlands)
TABLE 2 Traditional Chinese botanical drugs for the treatment of ALD and the regulation of oxidative stress effects

In addition, epigenetics and microbiota were also associated with the development of UF (Li et al., 2003

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