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In addition, a GSH/GSSG assay kit was used in this study, which is capable of accurately quantifying GSH/GSSG in cells by utilizing the enzymatic reaction between 5, 5-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase (GR)

Since the synthesis of NO by iNOS is elevated in inflammatory ailments and leads to cellular injury, this activity confirms its anti-inflammatory action (Won and Park, 2005)

Combined interventions are associated with complementary or additive improvements in hepatic steatosis, glycemic control, and inflammatory balance, particularly in metabolically compromised states

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