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Activated platelets (aPLT) were obtained by stimulating platelets with 2 U mL 1 thrombin and 8 nM CaCl 2 for 10 min at 37 C, The aPLT were then suspended in PBS containing 2% DMSO and frozen in liquid nitrogen for 12 h

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Reduced levels of oxygen may induce a high rate of cancer cells proliferation, generating areas where hypoxia can spread heterogeneously within the tumor mass, especially in solid tumors (132)

It has been demonstrated to possess a variety of biological activities, including substantial anti-inflammatory and anti-tumor properties, as well as the capacity to modulate immune function and inhibit the growth of pathogenic microorganisms (Li Y