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It is known that the production of ROS (reactive oxygen species) plays a role in protection against pathogenic microorganisms, but they can also cause tissue damage during this protection and trigger inflammation, where excess iron favors this process leading to cell death [2]

Class II (or purF-type) GATases, on the other hand, have a GD that adopts a four-layer fold and lacks a catalytic triad

It mitigates oxidative damage by scavenging free radicals and upregulating antioxidant enzymes, thereby preserving cellular homeostasis and preventing apoptosis [22,34,35,36,37]

However, the SAMP, N-(4-hydroxy-3-((2-oxoethyl)thio)phenyl)acetamide and 2-(5-Acetamido-2-hydroxyphenyl)sulfanylacetic acid metabolites, observed in the liver models and in vivo, could be generated in presence of all bacterial strains, and in some cases at higher levels compared to the liver models ( i.e., higher production of SAMP by E.coli and of N-(4-hydroxy-3-((2-oxoethyl)thio)phenyl)acetamide by E