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Membranes were blocked in 2% w/v skim milk powder dissolved in PBS (mPBS) and incubated for 4 h with horseradish peroxidase (HRP)-conjugated polyclonal sheep anti-HSA (Abcam, #ab8941) diluted 1:2000 in 2% mPBS and washed 4 times in PBS, before developing with TMB substrate (Sigma, #T0565) for membranes

10.1038/224117a0 [DOI] [PubMed] [Google Scholar] 15.Ballatori N, Krance SM, Notenboom S, Shi S, Tieu K, Hammond CL

Generally, phase II reaction increase the hydrophilicity of drugs but the acetylation does not increase hydrophilicity

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