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Description
This extended single dose toxicity study is the minimal requirement to perform Phase 0 clinical trials in humans
Lambs are more susceptible than calves
In particular, the addition of vitamin C to the culture medium showed an increased production of L-DOPA and dopamine, mainly due to both an enhancement at metabolic level and involving the gene expression of the enzymes responsible for producing L-DOPA and dopamine
The k cat and K m values of S586A toward tyrosine and DOPA are 250.9 4.2 s 1 and 0.42 0.03 mM and 126 3.48 s 1 and 0.69 0.07 mM, respectively
