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In cases of diabetic nephropathy, chronic hyperglycemia induces the formation of autoantibodies against podocyte antigens, which cross-react with glomerular components, triggering complement activation and foot process effacement

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Instrument parameters were set as follows: capillary voltage 3000 V, sample cone voltage 35 V, source temperature 110 C, desolvation temperature 320 C, desolvation gas flow 600 L/h, cone gas flow 15 L/h, extraction cone voltage 3.0 V, and collision energy 6 eV