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To construct the pPapi-SLC25 library, SpCas9 and SaCas9 guides were designed to target 53 SLC25 carriers, seven additional SLC proteins present in MitoCarta 2.0 16 , BCL2L1, MCL1 (the two paralogous anti-apoptotic proteins) and EEF2 (elongation factor 2, essential for protein translation) as positive controls, 8 non-cutting controls and 19 cutting controls that target olfactory receptors (ORs) as negative controls

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Their internal in vitro study using human kidney epithelial cells (786-O) demonstrates the superior performance of LiposoMax Glutathione compared to standard forms

To imitate hemorrhage toxicity in vitro , the model was constructed by applying 100 M hemin to Astrocytes ( Synthetic RNA Oligonucleotides and Transfection We purchased miRNA-146a-3p mimics, miRNA-146a-3p inhibitors, and a non-sense sequence for use as a miRNA negative control (NC) from Genepharma (Shanghai, China) (Supplementary Table 2)