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How to Use DIRECTIONS TO USE Moisten the suppositories with cold water, Insert deep into the rectum using fingers

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For measuring G s signaling, a plasmid mixture consisting of 200 ng LgBiT-inserted G s subunit, 1000 ng G1, 1000 ng C68S-mutant SmBiT-fused G2 (C68S), 400 ng test GPCR in 200 L of Opti-MEM (Gibco) was transiently transfected with PEI