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However, translating this growing knowledge into reliable diagnostics and effective therapies remains challenging

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GAPDH western blot was repeated twice and corroborated by proteomics experiments

Optical density (OD)/wavelength was quantified using a UV-1601 spectrometer (Shimadzu, Japan), and an ELISA plate reader was used to estimate antioxidant status, protein expressions, and inflammation ( 2.4 Assessment of lipid peroxidase Lipid peroxidation (LPO) was determined at pH 7.4 using the following reaction mixture: 0.2 mL of supernatant, 0.2 mL of 100 mM ascorbic acid, 0.58 mL of 0.1 M phosphate buffer (PB), and 0.02 mL of 100 mM ferric chloride