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Immobilization of curcumin on epoxy-sepharose beads and a pull-down assay Epoxy-activated sepharose beads (E6754 Sigma) were incubated with 20 mM curcumin in coupling buffer (50% dimethylformamide, 0.1 M Na 2 CO 3 , and 10 mM NaOH) at 37 C overnight in the dark, and then with 1 M ethanolamine (pH 11) at 37 C overnight to block the remaining non-specific binding sites

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[ 41 ] In contrast, ubiquitinspecific peptidase 47 (USP47) directly binds to BACH1, deubiquitinates and stabilizes BACH1 for the promotion of Warburg effect and the progression of nonsmall cell lung cancer