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JTCVS Open 10:296-297, 2022

doi: 10.1074/jbc.M308215200

Hua D, Yang Q, Li X, Zhou X, Kang Y, Zhao Y, et al

The 20 L reactions contained 12.5 L of TB Green Premix Ex Taq II (Tli RNaseH Plus), 0.4 L of ROX Reference Dye II, 200 ng of cDNA template, 0.4 L of each primer (10 M), and 6.26 L of PCR-grade water
