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J Trace Elem Med Biol 2014
the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

Furthermore, IL6bilirubin interaction, GSTM2butyric acid (butyrate) interaction, GPX3butyric acid (butyrate) interaction, and ACADMoctanoylcarnitine (octanoylcarnit.) interaction were identified with high confidence scores (0.7 or 70%)

Then, for T4 as a target, one of the future research directions is the development of related drugs as well as the ongoing, in-depth study of the mechanism of T4 promoting LC
