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The slides were finally mounted in PBS:Glycerol (Fischer chemical, #G33) solution (1:1), let sit for 10 min at room temperature and sealed using dots of Permount Mounting Medium on the edge of the coverslip (Corning, #2980-225)

According to molecular dynamics simulations, membrane oxidation, subsequent thinning, and an increase in curvature are facilitated by enhanced membrane curvature and a reduction in membrane thickness, enabling the penetration of oxidants into the membrane

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The interaction with eNOS and COX2 gene expression, and counteraction of the aggravation effect of the NOS-blocker, L-NAME [38], might suggest that NO system function might be accordingly recovered