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Briefly, cells were incubated at 37 C for 30 min with Apopxin Green for detection of phosphatidylserine on cell surface, propidium iodide (PI) or 7-ADD for labeling the nucleus of cells with membrane rupture, and CytoCalcein for labeling live cell cytoplasm

3.2.3 Application of PCLS to study cell death pathways Various cell death pathways have been observed in lung IRI, including apoptosis, necrosis, pyroptosis and ferroptosis ( 4 Summary, limitations and future perspectives 4.1 Summary Lung IRI is the main risk factor for primary graft dysfunction and mortality following LTx

Additionally, it has a short half-life (~ 7 days), which may contribute to higher rates of treatment failure [54, 55]

From branch to bedside: Youyou Tu is awarded the 2011 Lasker~DeBakey Clinical Medical Research Award for discovering artemisinin as a treatment for malaria