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Further studies using GFP-fusion promoter reporter strains revealed, that from the three, only mir-53 promoter activity was reduced by starvation in a sir-2.1 - and hsf-1 - dependent manner, while mir-75 promoter activity was maintained by HSF-1 and dropped in sir-2.1(RNAi) -treated starved worms to a similar level to that of hsf-1(RNAi) -treated animals (Figure 4C, D)