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In vitro treatment of CD38-overexpressing CHO cells (CHO-mCD38) with Ab68, but not Ab69, significantly increased both cellular NAD + levels and Sirt1 and Sirt3 deacetylase activity (Fig

However, when the assay was repeated with 5-amino-6-fluoro-1MQ, an analogue of 5-amino-1MQ that did not interfere with the NAMPT assay, no inhibition of NAMPT was observed with analogue concentrations between 30 and 600 M (data not shown)
