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Significant main effects of treatment group (prior to BenjaminiHochberg correction since none were significant after correction, although the necessity of correction for multiple comparisons in exploratory analyses remains unresolved 49 ) were observed for the unclassified group and ten classified genera, namely, Acetatifactor , Akkermansia , Erysipelatoclostridium , [ Eubacterium ] coprostanoligenes group , Family XIII UCG-001 , Lactobacillus , Parasutterella , Ruminiclostridium 6 , Ruminococcaceae UCG-004 , and Ruminococcaceae UCG-009

Mechanistic synergy: GLP-1 agonists such as semaglutide slow gastric emptying, suppress glucagon, and increase insulin, while cagrilintide enhances satiety and further slows digestion via amylin pathwaystogether providing complementary effects (The Innovation

Exact dosing varies by experimental design and species but generally follows preclinical trial standards for NNMT inhibitors

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