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The supernatants may be pooled or analyzed separately by SDS-PAGE to monitor protein content (see Note 12)
The sum of fat oxidation during the 1 h period averaged 13% higher in the EX group than in the CON and EXIN groups (data not shown)

Overall energy and immunity boost

Finally, MBP-His-SDJ1 was added to each tube and incubated in PBSN buffer at 4 C for 2 h