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To determine cell viability, a 50% Cell Titer Glo and 50% cell culture medium was added to each well and incubated at room temperature for 10 min

doi: 10.1093/eurheartj/ehm267

Ekman, A

Because the fat sits in the stomach for an extended period, it relaxes the lower esophageal sphincter, leading to severe acid reflux, dumping syndrome symptoms, and prolonged bouts of nausea
