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Description
The two engineered parental antibodies, each containing a single amino acid change in CH3 are first expressed separately, and then recombined using a reducing agent which mediates half-IG exchange

Smyth LCD, Highet B, Jansson D, Wu J, Rustenhoven J, Aalderink M, et al

But they are a strong signal that even when youre doing everything right, aggressive stacking carries hidden risk

Protein-protein interaction among the FoxP family members and their regulation of two target genes, VLDLR and CNTNAP2 in the zebra finch song system
