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The cultures were collected, their growth measured at optical density (OD) of 600 nm (Spectronic Helios Gamma UV-Vis Spectrophotometer, Thermo Fisher Scientific, UK), centrifuged at 3500 g for 1 min, and the supernatant was collected for measuring TMA, TMAO, BB, carnitine, and choline concentration using LC-MS

doi: 10.1042/BSR20160244

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