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Following 24, 48, 72, or 96 h after seeding, the medium was supplemented with 10 L of CCK-8 reagent (Beyotime) and the cells were incubated at 37 C for 2 h

J Food Sci 76, C52C58

Calycosin has been reported to play a role in preventing osteoporosis in postmenopausal women

F., Neuman, M
