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Together, these findings highlight the functional link between GLR-mediated Ca 2+ /NO signalling and mitochondrial stress regulation in plants, suggesting that Arabidopsis can serve as a tractable system to explore conserved principles of mitochondrial dysfunction relevant to neurodegeneration

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For quantitative assessment of cells, a segmentation mask was generated based on the staining for CD45 and nuclei (Propidiumiodide), which was then used to extract singlecell expression data from images of all measured markers (Fig EV1)