glutathione analysis method A Simple HPLC-UV for the Determination of in PC-12 Cells Glutathione Quantification Total Glutathione Quantification

Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g at 0 C), and the obtained supernatants was evaporated and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)

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Current Pharmaceutical Design, 20(8), 1138-1145

H 2 O 2 is produced during the SOD1-catalyzed oxidation of thiol compounds The toxicity of CSH and Hcy toward various cell types in the presence of free Cu 2+ (as CuCl 2 ) has been previously demonstrated and ascribed to the toxicity of H 2 O 2 and/or of hydroxyl radicals produced during Cu 2+ -catalyzed thiols oxidation 46,47,48,49,50