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Cellular proteins were extracted with extraction buffer (50 mM Tris pH 8.0, 500 mM NaCl, 10 mM -mercaptoethanol, and 10% glycerol), and affinity purification was performed using GST-accept resin (Nakarai)

[114, 115, 116, 117] Similarly, magnetic nanoparticles (MNPs) coated with a lupus-specific peptide have been shown to selectively target lupus B cells and induce immune tolerance

To investigate whether non-pathogenic extracellular materials elicit a similar response, we generated cellular debris from Caco2 colonic epithelial cells and exposed macrophages to them
