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Cell debris was removed by centrifugation at 10 000 g for 10 min and supernatants were sequentially passed through 46 PES filter membranes with the following range of pore sizes: 11, 6, 2.7, 1.7, 1.2, 0.7, 0.45, and 0.22 M

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Metabolic phenotypes do not correlate with microenvironment To exclude perfusion and hypoxia as explanations for the observed metabolic heterogeneity, we assessed cellular energy status from measurements of the ATP, ADP and PCr concentrations

Nanoparticles-based strategies to improve the delivery of therapeutic small interfering RNA in precision oncology