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The first-round PCR was performed in a 40 l volume, containing 1.0 g of template DNA, 1 l each of 10 M forward primer KI#169 and 10 M reverse primer KI#289, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no
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N-acetylcysteine ameliorates hyperoxic lung injury in the preterm guinea pig
