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To elucidate the underlying mechanism, they attempted to ionize phenol-containing dioxetanes and found that P3OT is a promising negative ion mode MALDI matrix

Structural analysis using AlphaFold multimer CBS sequences were obtained from UniProt 63 , and their sequences aligned using Clustal Omega 64

Materials and Methods Forty male albino mice of 20 weeks age at weights ranged between (28 and 32 g) were obtained from Charles River Laboratories/USA, hosted in plastic cages at temperature (20C28C) and dieted a basal diet according to Vodopich and Moore[] and accordance with the guidelines approved by the Animal Ethical Committee of University of Baghdad

Total DNA was extracted with Genomic DNA Isolation Kit (Norgen Biotek Corp., Thorold, Ontario, Canada), according to the manufacturers protocol