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This study was conducted on tissue cultures including W-256 (a rat breast cancer cell line), L1210 (a mouse lymphatic leukemia cell line), J111 (a human leukemia cell line), liver epithelial and liver fibroblasts of rats, skin fibroblasts of mice, and human breast and prostate cells that were normal or malignant [71]

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Researchers set out to find an adequate dose at that age, and it seems we need at least about 650 to 1,000 a day in most people, hence my 1,000-a-day recommendation after age 65