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H2DCFDA can penetrate the cell and convert to a non-fluorescent compound by intracellular esterase, and then to a highly fluorescent compound 2,7-Dichlorofluorescein (DCF) through oxidation
Researchers investigate receptor binding, intracellular signaling, and metabolic pathway responses associated with this concurrent triple activation, comparing it with single- and dual-agonist profiles in controlled laboratory settings [2]

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